Vista7 slic loader 2.4.9
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monocytogenes levels decreased by 1.3, 1.9, and 1.8 log 10 CFU/ham within 24 h at 4 ☌ in samples treated with 4, 6, and 8 mL of a 1:2 solution of ACS, respectively, compared to control hams that were not treated. For hams inoculated with 3.0 log 10 CFU, L. 2.0–5.0 log 10 CFU/ham in samples treated with 4, 6, and 8 mL of a 5% solution of LAE. Thereafter, pathogen levels remained relatively unchanged (☑.0 log 10 CFU/ham ) after 60 days at 4 ☌ in hams treated with 4, 6, and 8 mL of a 1:2 solution of ACS and increased by ca. monocytogenes levels decreased by ca.1.2, 1.5, and 2.0 log 10 CFU/ham and 5.1, 5.4, and 5.5 log 10 CFU/ham within 24 h at 4 ☌ in samples treated with 4, 6, and 8 mL of a 1:2 solution of ACS and a 5% solution of LAE, respectively, compared to control hams that were not treated with either antimicrobial. For hams inoculated with 7.0 log 10 CFU, L. monocytogenes, added to shrink-wrap bags that contained 0, 4, 6, or 8 mL of either a 1:2 solution of ACS or a 5% solution of LAE, vacuum-sealed, and stored at 4 ☌ for 60 days. The efficacy of ACS and LAE were further evaluated in shelf-life studies wherein hams were surface inoculated with either ca. 6.5 log 10 CFU/ham for all application volumes tested. In samples treated with 2, 4, 6, and 8 mL of a 5% solution of LAE, pathogen levels decreased by 3.3, 6.5, 5.6, and 6.5 log 10 CFU/ham, whereas when treated with a 10% solution of LAE pathogen levels decreased ca. Pathogen levels decreased by 1.2, 1.6, 2.4, and 3.1 log 10 CFU/ham and 0.7, 1.6, 2.2, and 2.6 log 10 CFU/ham in samples treated with 2, 4, 6, and 8 mL of a 1:1 and 1:2 solution of ACS, respectively. 7.0 log 10 CFU per ham), added to shrink-wrap bags that already contained ACS or LAE, vacuum-sealed, and stored at 4 ☌ for 24 h. Hams were surface inoculated with a five-strain cocktail of L.
Vista7 slic loader 2.4.9 plus#
The pathogen was Listeria monocytogenes, the antimicrobials were acidic calcium sulfate (ACS calcium sulfate plus lactic acid 1:1 or 1:2 in dH 2O) and lauric arginate (LAE Ethyl- N-dodecanoyl- l-arginate hydrochloride 5% or 10% in dH 2O), and the product was commercially prepared “table brown” ham (ca. We demonstrated the effectiveness of delivering an antimicrobial purge/fluid into shrink-wrap bags immediately prior to introducing the product and vacuum sealing, namely the “Sprayed Lethality In Container” (SLIC™) intervention delivery method.